A multilayer film of chitosan, 3.5 nm negatively charged CdSe quantum dots, and organophosphorus hydrolase was deposited onto quartz slide via the layer-by-layer deposition technique of polyelectrolytes. Concentration of paraoxon in solution was correlated to UV-Vis absorbance of the hydrolytic product p-nitrophenol and photoluminescence quenching of the quantum dots.
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