kerimistry
LOCATION:
GAINESVILLE, FL
EMPLOYER:
UNIVERSITY OF FLORIDA
Kerim M. Gattás Asfura, Ph.D.
Chemist.
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Member of:
October 17, 2018

Perfluorinated Alginate Beads

Scaffolds for Tissue Engineering / Hydrogels

Alginate is a natural polysacharide for use in cellular encapsulation. Perfluorocarbons have unique chemical and physical properties some of which can find biological-relevant applications. The incorporation of perfluorocarbons emulsions has been utilized to enhance oxygenation through cell-containing capsules. Perfluorocarbons can also be utilized to influence binding interactions at surfaces. For applications to treat diabetes, pancreatic islet transplantation is considered one promising approach. Pancreatic islets require high demand of oxygen and should be protected from immune attact for use in islet transplantation. Hence, perfluorocarbon chains were covalently linked to alginate's polymeric backbone via a poly(ethylene glycol) linker. The use of covalent bonds localize the fluorinated chains within the scaffolds via a surfactant-free approach. Being the fluorinated chains incorporated at the molecular level, it was reasoned that self-assembly of the fluorinated chains may enhance oxygen solubility and diffusive permeability through the alginate scaffold and provide a more immuno-inert scaffold. Studies with insulin producing mouse insulinoma MIN6 cells revealed that MIN6 cells remained viable and proliferated significantly higher if compared to controls consisting of cells encapsulated within untreated alginate capsules. Enhanced viability resulted in part or combination from enhanced mass transport and unique mechanical and chemical properties of the resulting fluorinated hydrogels. Hence, the fluorinated biomaterials may find applications for cellular encapsulation in particular for the encapsulation of pancreatic islets.

Gattás-Asfura, K. M.; Fraker, C. A.; Stabler, C. L. J. Biomed. Mater. Res. Part A 2012, 100A, 1963-1971.

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